Requirements for In Vitro Germination of Paenibacillus larvae Spores

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Detection of Paenibacillus larvae larvae spores in honey and diseased larvae Samples by culture and PCR

American foulbrood (AFB) is the most serious brood disease of the honey bee. Traditional methods are reliable but rather slow simply because they are based on biochemical, morphological and physiological identification of cultivated isolates. The aim of this study is the detection of Paenibacillus larvae larvae spores in honey and diseased larvae samples by culture and PCR. Therefore 54 samp...

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Requirements for germination of Clostridium sordellii spores in vitro.

Clostridium sordellii is a spore-forming, obligately anaerobic, Gram-positive bacterium that can cause toxic shock syndrome after gynecological procedures. Although the incidence of C. sordellii infection is low, it is fatal in most cases. Since spore germination is believed to be the first step in the establishment of Bacilli and Clostridia infections, we analyzed the requirements for C. sorde...

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detection of paenibacillus larvae larvae spores in honey and diseased larvae samples by culture and pcr

american foulbrood (afb) is the most serious brood disease of the honey bee. traditional methods are reliable but rather slow simply because they are based on biochemical, morphological and physiological identification of cultivated isolates. the aim of this study is the detection of paenibacillus larvae larvae spores in honey and diseased larvae samples by culture and pcr. therefore 54 samp...

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Germination requirements of Bacillus macerans spores.

2-Phenylacetamide is an effective germinant for spores of five strains of Bacillus macerans, particularly in the presence of fructose. Benzyl penicillin, the phenyl acetamide derivative of penicillin, and phenylacetic acid are also good germinants. l-Asparagine is an excellent germinant for four strains. alpha-Amino-butyric acid is moderately effective. Pyridoxine, pyridoxal, adenine, and 2,6-d...

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Requirements for production and germination of spores of anaerobic bacteria.

The important role of amino acids in spore germination was established by Hills (1949) and Stewart and Halvorson (1953). Their studies, however, were limited to the aerobic forms because the anaerobes could not be made to produce sufficient quantities of spores in media within a specified time. Using an autoclaved 1 per cent solution of glucose phosphate, Wynne et al. (1954) germinated anaerobi...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 2012

ISSN: 0021-9193

DOI: 10.1128/jb.01958-12